Molecular and phylogenetic analysis of Sardinella lemuru Bleeker 1835 at fishing ground Canggu-Bali inferred D-loop mutations of mtDNA
نویسندگان
چکیده
Abstract Sardinella lemuru resources are abundant, nutritious, high omega-3 and have economic value in artisanal fisheries. Its production volume landed for over a decade, but the most significant increase was peak southeast monsoon 2019 at Canggu fishing ground. However, prior-conventional kinship assessments genetically need to be confirmed. These research objectives encompass both molecular identification genetic relationship of S. with others. Similar morphological samples had collected ten individuals. Chelex 10% used genomic DNA extraction, CRK-CRE primer Hotstart-50 PCR thermal profile applied amplification. 1% agarose electrophoresis Sanger sequencing examined quantity quality D-loop amplicon. Identification using algorithm BLASTn. Phylogenetic tree analysis uses neighbor-joining method. The identity 10 (97.46%-98.47%) as Philippines (MK579633.1-MK579742.1). phylogenetic significantly shows low barrier inter-waters from moderate distance. Inter-waters very close intraclade, it still has different mutation rate another clade. Means suggested that is more diverse nucleotide base substitutions encouraging variation. study results provide needed information southern translocation occurred among waters, its dispersal connection.
منابع مشابه
Genetic Diversity and Phylogenetic Analysis of the mtDNA D-loop Region in Tibetan Sheep
Seventeen haplotypes were detected from the complete mitochondrial DNA control region sequences analyzed from eighty individuals of two Tibetan domestic sheep breeds. The nucleotide composition of all the sequences was 33.0% A, 29.7%T, 22.9%C and 14.4%G; G+C was 37.3%. The length of the sequences ranged from 1,107 bp to 1,259 bp. The difference between them was primarily due to 3-5 copy numbers...
متن کاملPhylogenetic Analysis of a Privately-owned Korean Native Chicken Population Using mtDNA D-loop Variations
The use of Korean native chicken is increasing, and the discovery of new genetic resources is very important from both economic and genetic conservation points of view. In this study, mtDNA D-loop sequences from 272 privately-owned Korean native chickens from a Hyunin farm were investigated. Seventeen nucleotide substitutions were identified from the sequence analysis and they were classified a...
متن کاملphylogenetic and genetic analysis of d-loop and cyt-b region of mtdna sequence in iranian sistani, sarabi and brown swiss cows
cattle have an important role in primary human civilization, so molecular studies for more accurate recognition of their origin are effective to identify unknown historical aspects. cattle can be divided in to 2 main groups including bos tuarus and bos indicus. both types of cattle can be found in iran; therefore study of their origin has particular importance. the aim of this study was to inve...
متن کاملSusceptibility of Sardinella lemuru to emerging marine microplastic pollution
Marine microplastics are emerging pollutants that impact across levels of marine food chain at a global scale. Its presence was determined on Sardinella lemuru, a commercial pelagic fish that are harvested generally in the Northern Mindanao, consumed locally, and exported worldwide as bottled or canned sardine products. The stomach contents of 600 sardines were examined visually under...
متن کاملlangauge needs analysis of undergraduate business management and economics students
the aim of conducting this study was to investigate the foreign language learning needs of undergraduate economics students and business management students in faculties of social sciences of alzahra and azad naragh university. in the study, which was designed on the basis of a qualitative-quantitative basis using interviews and questionnaires, 146 female undergraduate business management as we...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: IOP conference series
سال: 2022
ISSN: ['1757-899X', '1757-8981']
DOI: https://doi.org/10.1088/1755-1315/1036/1/012065